codon optimized cas9 (Addgene inc)
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Addgene inc
codon optimized cas9
Codon Optimized Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+codon+optimized+cas9/LentiV_Cas9_puro+(Plasmid+%23108100)/bio_rxiv__64898__2026__01__21__700714-205-16-22
Average 96 stars, based on 90 article reviews
Codon Optimized Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+codon+optimized+cas9/LentiV_Cas9_puro+(Plasmid+%23108100)/bio_rxiv__64898__2026__01__21__700714-205-16-22
Average 96 stars, based on 90 article reviews
codon optimized cas9 - by Bioz Stars,
2026-10
96/100 stars
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Related Articles
Plasmid Preparation:Article Title: A genetically modified minipig model for Alzheimer’s disease with SORL1 haploinsufficiency Article Snippet: .. The Article Title: In vivo evidence that SORL1 , encoding the endosomal recycling receptor SORLA, can function as a causal gene in Alzheimer’s Disease Article Snippet: .. The Article Title: Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs Article Snippet: VEGFA site#1 (‘ VEGFA sgRNA1’), VEGFA site#2 (‘ VEGFA sgRNA2’), and VEGFA Site#3 (‘ VEGFA sgRNA3’) were gifts from K. Joung, Massachusetts General Hospital (Addgene plasmids 47505, 47506, and 47507). .. An N-terminal FLAG tag sequence was appended via Gibson Assembly Cloning (New England Biosciences) to a Article Title: High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation Article Snippet: Cells were regularly passaged and tested for presence of mycoplasma contamination (MycoAlert Plus Mycoplasma Detection Kit, Lonza). .. In order to enable a meaningful comparison between different Cas9 variants, we used the Article Title: A genetically modified minipig model for Alzheimer's disease with SORL1 haploinsufficiency. Article Snippet: Successful gene targeting using the rAAV/SORL1 KO-Neo plasmid vector results in deletion of 609 bp in the endogenous porcine SORL1 gene including the entire exon 1. .. The CRISPR/Cas9 single-guide RNAs (sgRNAs) targeting the porcine SORL1 gene were designed employing the online designing tool ZiFiT.72 The Article Title: SNAT7 regulates mTORC1 via macropinocytosis Article Snippet: .. Guide RNA sequences for RRAGA and RRAGB were cloned into a bicistronic expression vector (pX459) containing Article Title: High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation Article Snippet: Cells were regularly passaged and tested for presence of mycoplasma contamination (MycoAlert Plus Mycoplasma Detection Kit, Lonza). .. In order to enable a meaningful comparison between different Cas9 variants, we used the FLAG-tag:Article Title: Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs Article Snippet: VEGFA site#1 (‘ VEGFA sgRNA1’), VEGFA site#2 (‘ VEGFA sgRNA2’), and VEGFA Site#3 (‘ VEGFA sgRNA3’) were gifts from K. Joung, Massachusetts General Hospital (Addgene plasmids 47505, 47506, and 47507). .. An N-terminal FLAG tag sequence was appended via Gibson Assembly Cloning (New England Biosciences) to a Sequencing:Article Title: Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs Article Snippet: VEGFA site#1 (‘ VEGFA sgRNA1’), VEGFA site#2 (‘ VEGFA sgRNA2’), and VEGFA Site#3 (‘ VEGFA sgRNA3’) were gifts from K. Joung, Massachusetts General Hospital (Addgene plasmids 47505, 47506, and 47507). .. An N-terminal FLAG tag sequence was appended via Gibson Assembly Cloning (New England Biosciences) to a Cloning:Article Title: Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs Article Snippet: VEGFA site#1 (‘ VEGFA sgRNA1’), VEGFA site#2 (‘ VEGFA sgRNA2’), and VEGFA Site#3 (‘ VEGFA sgRNA3’) were gifts from K. Joung, Massachusetts General Hospital (Addgene plasmids 47505, 47506, and 47507). .. An N-terminal FLAG tag sequence was appended via Gibson Assembly Cloning (New England Biosciences) to a Comparison:Article Title: High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation Article Snippet: Cells were regularly passaged and tested for presence of mycoplasma contamination (MycoAlert Plus Mycoplasma Detection Kit, Lonza). .. In order to enable a meaningful comparison between different Cas9 variants, we used the Article Title: High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation Article Snippet: Cells were regularly passaged and tested for presence of mycoplasma contamination (MycoAlert Plus Mycoplasma Detection Kit, Lonza). .. In order to enable a meaningful comparison between different Cas9 variants, we used the CRISPR:Article Title: A genetically modified minipig model for Alzheimer's disease with SORL1 haploinsufficiency. Article Snippet: Successful gene targeting using the rAAV/SORL1 KO-Neo plasmid vector results in deletion of 609 bp in the endogenous porcine SORL1 gene including the entire exon 1. .. The CRISPR/Cas9 single-guide RNAs (sgRNAs) targeting the porcine SORL1 gene were designed employing the online designing tool ZiFiT.72 The Clone Assay:Article Title: SNAT7 regulates mTORC1 via macropinocytosis Article Snippet: .. Guide RNA sequences for RRAGA and RRAGB were cloned into a bicistronic expression vector (pX459) containing Expressing:Article Title: SNAT7 regulates mTORC1 via macropinocytosis Article Snippet: .. Guide RNA sequences for RRAGA and RRAGB were cloned into a bicistronic expression vector (pX459) containing Transfection:Article Title: The C9ORF72 repeat expansion alters neurodevelopment. Article Snippet: .. 2 3 106 C9ORF72 ALS/FTD line 1 iPSCs were transfected with Construct:Article Title: The C9ORF72 repeat expansion alters neurodevelopment. Article Snippet: .. 2 3 106 C9ORF72 ALS/FTD line 1 iPSCs were transfected with Homologous Recombination:Article Title: The C9ORF72 repeat expansion alters neurodevelopment. Article Snippet: .. 2 3 106 C9ORF72 ALS/FTD line 1 iPSCs were transfected with |
